Device

Part:BBa_K4213049:Design

Designed by: Eleftheria Lakaki   Group: iGEM22_Thessaly   (2022-09-06)


TetO7-p35S-Venus-tNOS(stuffer)_pNOS-TetR-Krab-tNOS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 353
    Illegal NheI site found at 1515
    Illegal NheI site found at 3077
    Illegal PstI site found at 347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 353
    Illegal XhoI site found at 34
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 353
    Illegal PstI site found at 347
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GoldenBRaid 2.0


Source

-

References